MATRIX-ASSISTED LASER DESORPTION IONISATION MASS-SPECTROMETRY GUIDED PURIFICATION OF HUMAN GUANYLIN FROM BLOOD ULTRAFILTRATE/

Citation
M. Schrader et al., MATRIX-ASSISTED LASER DESORPTION IONISATION MASS-SPECTROMETRY GUIDED PURIFICATION OF HUMAN GUANYLIN FROM BLOOD ULTRAFILTRATE/, Journal of chromatography, 776(1), 1997, pp. 139-145
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
776
Issue
1
Year of publication
1997
Pages
139 - 145
Database
ISI
SICI code
Abstract
The purification of the human peptide hormone guanylin 22-115 from blo od ultrafiltrate (hemofiltrate, HF) was achieved using matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) as the assay system. Screening a peptide bank generated from 5000 l HF guanylin 22 -115 was detected by its molecular mass when adequate conditions for M ALDI-MS analysis were chosen. The sensitivity was even better than of the established biological assay system. In addition, the susceptibili ty towards solvents and salts is strongly reduced. 1.2 mg of the pepti de hormone was purified from 10% of the starting material. (C) 1997 El sevier Science B.V.