M. Schrader et al., MATRIX-ASSISTED LASER DESORPTION IONISATION MASS-SPECTROMETRY GUIDED PURIFICATION OF HUMAN GUANYLIN FROM BLOOD ULTRAFILTRATE/, Journal of chromatography, 776(1), 1997, pp. 139-145
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The purification of the human peptide hormone guanylin 22-115 from blo
od ultrafiltrate (hemofiltrate, HF) was achieved using matrix-assisted
laser desorption/ionisation mass spectrometry (MALDI-MS) as the assay
system. Screening a peptide bank generated from 5000 l HF guanylin 22
-115 was detected by its molecular mass when adequate conditions for M
ALDI-MS analysis were chosen. The sensitivity was even better than of
the established biological assay system. In addition, the susceptibili
ty towards solvents and salts is strongly reduced. 1.2 mg of the pepti
de hormone was purified from 10% of the starting material. (C) 1997 El
sevier Science B.V.