By using the in vitro Ames Salmonella/microsomal assay and the in vivo
mouse micronucleus assay, studies were performed to evaluate the geno
toxicity of gasoline exhaust particulate matter generated from five di
fferent domestic and imported scooters. In the Ames assay, treatment o
f test strains TA98 and TA100 with solvent extracts of particulate mat
ter from four of five scooter models caused an increase in the number
of histidine-independent colonies over the background in TA98 without
S9 mix. Positive results were also obtained from the micronucleus assa
y. The frequencies of bone marrow micronucleated polychromatic erythro
cytes were significantly higher in the treated compared to the nontrea
ted animals, and the increases in the frequencies were not significant
ly different among the five types of scooters. Analyses of chemical co
mponents showed that scooter exhaust particulate matter contained more
than 100 different substances including polycyclic aromatic hydrocarb
ons.