Mb. Hall et al., A SIMPLE METHOD FOR ESTIMATION OF NEUTRAL DETERGENT-SOLUBLE FIBER, Journal of the Science of Food and Agriculture, 74(4), 1997, pp. 441-449
A method was developed to estimate the neutral detergent-soluble fibre
(NDSF) content of feeds. Citrus pulp, sugar beet pulp, soybean hulls,
separated leaves and stems from mature and immature alfalfa, red clov
er, reed canarygrass and timothy were analysed. Half-gram samples were
stirred at room temperature for 4 h with 100 ml of 90:10 (v/v) ethano
l/water to extract low molecular weight substances, crude protein (CP)
and ash to produce ethanol-insoluble residues (EIR). Sampies were ref
luxed for Ih with neutral detergent solution and heat stable alpha-amy
lase to extract low molecular weight substances, NDSF, starch, CP and
ash to produce neutral detergent residues (NDF). The EIR and NDF were
analysed for organic matter (OM; EIROM; NDFOM) and CP (EIRCP; NDFCP),
and EIR was analysed for starch. Values were expressed as proportions
of the original sample dry matter. The NDSF was calculated as the diff
erence in OM mass between EIR and NDF after correction for CP and star
ch by the equation: EIROM - NDFOM - EIRCP + NDFCP - EIR starch. Extrac
tions with 90:10 (v/v) ethanol/water gave a higher yield of EIR and ND
SF than did 80:20 (v/v) ethanol/water. The method gave good precision
and is convenient for assessing NDSF content of feeds.