Am. Harsulkar et al., PROTEASE INHIBITORS OF CHICKPEA (CICER-ARIETINUM L) DURING SEED DEVELOPMENT, Journal of the Science of Food and Agriculture, 74(4), 1997, pp. 509-512
Developing seeds of two chickpea varieties were collected on the 20th,
40th and 60th day after flowering (DAF) and analysed for trypsin inhi
bitors (TI). The varieties differed in their TI profiles and activity
units. The TI band and activity were not detectable at 20 DAF in the v
arieties Annigeri and BDN 9-3. Annigeri showed the highest total and s
pecific TI activity, and five isoinhibitors at 40 DAF, which decreased
to four at 60 DAF. The appearance and disappearance of TI bands durin
g seed development indicate their specific posttranslational modificat
ion and/or the presence of isolated gene groups.