POLYMERIC ADSORBENTS WITH PEPTIDE PENDANTS AS ARTIFICIAL RECEPTORS FOR BETA-LACTAM ANTIBIOTICS BY MIMICKING THE BINDING-SITES OF BETA-LACTAMASES

A series of polymeric adsorbents with peptide pendants were designed a s the artificial receptors of beta-lactam antibiotics by mimicking the structures of binding site in beta-lactamases. Crosslinked poly(N, N- dimethyl acrylamide) gel as a carrier was prepared by suspension copol ymerization of N, N-dimethyl acrylamide and N, N'-bisacryldiaminoethan e and then functionalized with ethylenediamine after partial hydrolysi s. Using solid-phase peptide synthesis with symmetrical anhydride of p rotected amino acid step by step, various peptide pendants were respec tively anchored onto the functionalized carrier. The adsorption proper ties of these peptide-containing adsorbents for beta-lactam antibiotic s such as ampicillin and cefotaxime were then studied. The results sho wed that only those adsorbents in which peptide chains contained more than one lysine residues could obviously adsorb both beta-lactams and that static interaction as well as hydrogen bond played an important r ole during the adsorption.