Tg. Schlagheck et al., OLESTRA EFFECT ON VITAMIN-D AND VITAMIN-E IN HUMANS CAN BE OFFSET BY INCREASING DIETARY LEVELS OF THESE VITAMINS, The Journal of nutrition, 127, 1997, pp. 1666-1685
One hundred two normal healthy males and females were given 0, 8, 20 o
r 32 g/d olestra to which had been added graded amounts of vitamins A,
D and E for 8 wk in a parallel, double-blind study. The primary purpo
se of the study was to determine the amounts of vitamins D and E neede
d to offset the effect of olestra on the availability of these vitamin
s. Serum concentrations of retinol, carotenoids, 25-hydroxyvitamin D m
etabolites, alpha-tocopherol, phylloquinone, lipids, ferritin and tota
l iron, iron-binding capacity and hematology parameters, plasma concen
trations of des-gamma-carboxyprothrombin and prothrombin, and urinary
gamma-carboxyglutamic acid (Gla) excretion were measured biweekly. Cli
nical chemistry and urinalysis parameters, vitamin B-12 absorption, an
d serum 1,25-dihydroxyvitamin D concentration were measured at wk 0 an
d 8. Serum concentrations of alpha-tocopherol and 25-hydroxyergocalcif
erol were restored to control concentration by adding 2.1 mg d-alpha-t
ocopheryl acetate and 0.06 mu g ergocalciferol per gram of olestra, re
spectively, to the diet. Olestra reduced serum concentrations of 25-hy
droxyergocalciferol, carotenoids and phylloquinone in a dose-responsiv
e manner but did not affect Gla excretion, plasma des-gamma-carboxypro
thrombin and prothrombin concentrations, overall vitamin D status, vit
amin B-12 absorption or iron status. Laboratory evaluations showed no
olestra-related effects. Subjects in all groups reported mild to moder
ately severe transient gastrointestinal symptoms. These symptoms did n
ot affect study compliance or the integrity of the data.