M. Anwar et M. Masoomyasinzai, DETERMINATION OF FREE STEROL CONTENT OF VARIOUS BRANDS OF EGGS, GHEE OILS, USING IMMOBILIZED ENZYME COLUMN IN A FLOW SYSTEM/, Journal of the Chemical Society of Pakistan, 19(1), 1997, pp. 31-33
A flow system containing immobilized cholesterol oxidase column is app
lied to the analysis of various brands of eggs, ghee, oil etc., for th
e free sterol content. Eggs of domestic hens have significantly low co
ntent of free cholesterol as compared to cholesterol level in farmy eg
gs. The total sterol content of the ghee is lower as compared to oils,
probably because of the loss of sterol during bleaching, blending and
refining. The sterol content varies widely among different oils/ghee.