A. Maenpaa et al., LYMPHOCYTE ADHESION MOLECULE LIGANDS AND EXTRACELLULAR-MATRIX PROTEINS IN GLIOMAS AND NORMAL BRAIN - EXPRESSION OF VCAM-1 IN GLIOMAS, Acta Neuropathologica, 94(3), 1997, pp. 216-225
To identify antigenic differences between gliomas and normal brain. we
have immunohistochemically studied the expression of lymphocyte adhes
ion molecules (ICAM-1, ICAM-2. ICAM-3, VCAM-1, E-selectin and CD58), e
pidermal growth factor receptor (EGFR) and extracellular matrix protei
ns (collagen IV, fibronectin, laminin, merosin. tenascin and vitronect
in) in these tissues. Gliomas expressed high levels of ICAM-1. CD58 (L
FA-3), EGFR, tenascin and vitronectin, whereas only very low levels we
re detected in normal brain, VCAM-1 expression was detected in 15 out
of 25 gliomas but not in normal brain. The presence of VCAM-1 in gliom
as was verified by immunoblotting and RNase protection assay: and in g
lioma cell lines by Northern blotting. Expression of VCAM-1 in gliomas
may partially explain lymphocytic infiltration, and anti-VCAM-1 antib
odies may be of potential in antibody mixtures for targeted therapy of
gliomas.