SUPPRESSED FORMATION OF BONE-MARROW ADHERENT LAYERS DERIVED FROM ACUTE MYELOID-LEUKEMIA PATIENTS AFTER IN-VITRO EXPOSURE TO INTERLEUKIN-4

Long-term bone marrow cultures from ten acute myeloid leukemia (AML) p atients exposed to recombinant human (rhu) interleukin (IL) 4 from cul ture initiation failed to develop adherent layers at 5 weeks as compar ed to controls. The adherent layers from two of our patients expressed IL-1 beta transcripts constitutively, and all produced IL-6 and leuke mia inhibitory factor (LIF) proteins. Our results demonstrate that rhu lL-4 markedly inhibited AML-derived adherent layer formation in a time -and dose-dependent manner, and this effect was not mediated through e nhanced apoptosis and did not correlate with IL-1 beta, IL-6 or LIF pr oduction. (C) 1997 Elsevier Science Ltd.