DISCRETE INTENSITY JUMPS AND INTRAMOLECULAR ELECTRONIC-ENERGY TRANSFER IN THE SPECTROSCOPY OF SINGLE CONJUGATED POLYMER-MOLECULES

Single-molecule fluorescence spectroscopy of a multichromophoric conju gated polymer (molecular weight similar to 20,000) revealed surprising single-step photobleaching kinetics and acute jumps in fluorescence i ntensity. These jumps were shown not to result from spectral diffusion and were attributed to fluctuations in the quantum yield of emission for the molecules. The data indicate efficient intramolecular electron ic energy transfer along the polymer chain to a localized fluorescence -quenching polymer defect. The defects are created by reversible photo chemistry of the polymer. These findings have implications for the use of conjugated polymers in light-emitting diode displays and sensors.