APPLICATION OF NEAR-INFRARED SPECTROSCOPY TO THE DETERMINATION OF HEMOGLOBIN

Seventy-two whole blood samples were investigated to determine the rel ationship between their spectral data measured in the near infrared (N IR) wavelength region and haemoglobin content based on laboratory data determined by a routine standard method as reference. Blood samples w ere obtained from the Ist Department of Medicine, Imre Haynal Universi ty of Health Sciences. Donors were selected randomly without respect t o age, sex, state of health or medical treatment, from apparently heal thy volunteers as well as from ambulatory and hospitalized patients. N IR spectra were measured with a SPECTRALYZER 1025 (PMC) computerized s pectrophotometer in the 1000-2500 nm wavelength region. The relationsh ip between laboratory data and values of the second derivative (i.e. s econd order finite difference) of the log(1/TF) spectra measured at di fferent wavelengths was determined by multiple linear regression (MLR) using three-and four-term linear summation equations. The cross-valid ated standard error of performance (SEP) for haemoglobin was 1.348 g d L(-1) with a three term model acid 1.251 g dL(-1) with a four term mod el over the range from 5.9 to 20 g dL(-1). This preliminary study indi cates that NIR measurements can be directly related to haemoglobin con tent and can be used to determine haemoglobin content in human whole b lood. (C) 1997 Elsevier Science B.V.