EFFECTS OF ESCULETIN AS A LIPOXYGENASE INHIBITOR IN SOYBEAN EXTRACTS

This study was designed to determine the ability of esculetin to inhib it lipoxygenase activity in soybean. Lipoxygenase was extracted from g round soybean using 0.1 M Tris-HCl buffer (pH 8.6) and centrifuged at 3000 g for 15 min at 4C. The extract was mixed with different volumes of 0.05M esculetin solutions to provide 1.8 x 10(-4) M, 3.3 x 10(-4) M , 4.6 x 10(-4) M and 5.7 x 10(-4) M to final assay systems, respective ly. Lipoxygenase activity decreased as the concentration of esculetin solution mixed in soybean extract increased, Mixing of 1.8 x 10(-4) M esculetin solution to soybean extract showed the least inhibition effe ct and was significantly different from that of 3.3 x 10(-4) M, 4.6 x 10(-4) M and 5.7 x 10(-4) M which inhibited LOX (lipoxygenase)'s activ ity of soybean extracts. In the study comparing the inhibition abiliti es for lipoxygenase activity among selected antioxidants, esculetin wa s significantly better than BHA (butylated hydroxyanisole) and alpha-t ocopherol.