DIFFERENT BINDINGS OF THE MINOR-GROOVE LIGANDS DAPI AND HOECHST-33258TO MULTIMERS OF THE CURVED (CA(4)T(4)G) AND NONCURVED (CT(4)A(4)G) DNA-SEQUENCES

Authors
CANEVA RC DESIMONI A MAYOL L ROSSETTI L SAVINO M
Citation
Rc. Caneva et al., DIFFERENT BINDINGS OF THE MINOR-GROOVE LIGANDS DAPI AND HOECHST-33258TO MULTIMERS OF THE CURVED (CA(4)T(4)G) AND NONCURVED (CT(4)A(4)G) DNA-SEQUENCES, Biochimica et biophysica acta, N. Gene structure and expression, 1353(2), 1997, pp. 93-97
Citations number
31
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1353
Issue
2
Year of publication
1997
Pages
93 - 97
Database
ISI
SICI code
0167-4781(1997)1353:2<93:DBOTML>2.0.ZU;2-N
Abstract
Equilibrium binding properties of DAPI and Hoechst for prototype curve d (CA(4)T(4)G)(n) and straight (CT(4)A(4)G)(n) DNAs were derived from fluorescence intensity. Both decamers display a single strong binding site for each ligand. Hoechst binds seven times stronger to curved tha n to straight DNA. An additional cooperative, much weaker binding site is found for DAPI. (C) 1997 Elsevier Science B.V.