GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC IDENTIFICATION OF MAIN METABOLITES OF STANOZOLOL IN CATTLE AFTER ORAL AND SUBCUTANEOUS ADMINISTRATION

Authors
FERCHAUD V LEBIZEC B MONTRADE MP MAUME D MONTEAU F ANDRE F
Citation
V. Ferchaud et al., GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC IDENTIFICATION OF MAIN METABOLITES OF STANOZOLOL IN CATTLE AFTER ORAL AND SUBCUTANEOUS ADMINISTRATION, Journal of chromatography B. Biomedical sciences and applications, 695(2), 1997, pp. 269-277
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
695
Issue
2
Year of publication
1997
Pages
269 - 277
Database
ISI
SICI code
0378-4347(1997)695:2<269:GMIOMM>2.0.ZU;2-T
Abstract
An analytical method has been developed in order to control the illega l use of stanozolol as growth promoter in livestock. The procedure was based on enzymatic hydrolysis, purification on a Clean Screen DAU col umn and derivatization with heptafluorobutyric anhydride prior to GC-M S analysis. This method allowed us to study the metabolism of stanozol ol in cattle after oral and subcutaneous administrations. Urinary meta bolites were identified by mass spectrometry. Stanozolol and 16-hydrox ystanozolol were detected after oral administration, while 16-hydroxys tanozolol and 4,16-dihydroxystanozolol were found after subcutaneous a dministration.