DETERMINATION OF ZD9583, A THROMBOXANE RECEPTOR ANTAGONIST, IN HUMAN PLASMA AND URINE BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION TANDEM MASS-SPECTROMETRY
Cx. Gao et al., DETERMINATION OF ZD9583, A THROMBOXANE RECEPTOR ANTAGONIST, IN HUMAN PLASMA AND URINE BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION TANDEM MASS-SPECTROMETRY, Journal of chromatography B. Biomedical sciences and applications, 695(2), 1997, pp. 299-307
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A liquid chromatography-atmospheric pressure chemical ionization tande
m mass spectrometry (LC-APCI-MS-MS) method is described for the determ
ination of a thromboxane receptor antagonist thylethyl)-4-(3-pyridyl)-
3-dioxan-5-yl)hex-4-enoic acid (ZD9583, I) in human plasma and urine.
Proteins in plasma and urine samples are precipitated using acidified
acetonitrile. The resulting supernatant is chromatographed on a C-8 re
versed-phase chromatography column. Following the diversion of the sol
vent front from the mass spectrometer by a switching valve, the column
eluate is passed on to the mass spectrometer via a heated nebulizer i
nterface where the analyte is detected by multiple reaction monitoring
(MRM). The method has a chromatographic run time of less than 2 min,
a linear calibration curve with a range of 1-500 ng ml(-1) and intra-a
nd inter-day precision estimates of less than 10% over the calibration
range. (C) 1997 Elsevier Science B.V.