HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MEASUREMENT OF UROCANIC ACID ISOMERS AND THEIR RATIOS IN NATURALLY LIGHT-EXPOSED SKIN AND NATURALLYSHIELDED SKIN
K. Shibata et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MEASUREMENT OF UROCANIC ACID ISOMERS AND THEIR RATIOS IN NATURALLY LIGHT-EXPOSED SKIN AND NATURALLYSHIELDED SKIN, Journal of chromatography B. Biomedical sciences and applications, 695(2), 1997, pp. 434-438
Citations number
26
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
We have developed methods for sampling and extraction of trans-urocani
c acid and cis-urocanic acid from human skin, and subsequent high-perf
ormance liquid chromatographic measurement of these isomers. Sampling
involves applying cellophane adhesive tape to the skin for 10 s. Uroca
nic acid isomers were completely extracted by immersing the tape in KO
H solution. The HPLC column was a Tosoh ODS 80(TS) (250x4.6 mm LD., 7
mu m average particle size) eluted with 20 mM potassium dihydrogenphos
phate containing 1 g/l sodium heptanesulphonate (pH 3.7)-acetonitrile
(93:7, v/v) at a flow-rate of 1.0 ml/min. The isomers were detected by
UV absorbance at 264 nm. This technique was used to analyze the ratio
of trans-urocanic acid/cis-urocanic acid on human skin at various sit
es on the body. It was found that the ratio was low in naturally Light
-exposed skin and high in naturally shielded skin. (C) 1997 Elsevier S
cience B.V.