DIAFILTRATION IN THE PRESENCE OF ASCORBATE IN THE PURIFICATION OF MUSHROOM TYROSINASE

Authors
RESCIGNO A SOLLAI F SANJUST E RINALDI AC CURRELI N RINALDI A
Citation
A. Rescigno et al., DIAFILTRATION IN THE PRESENCE OF ASCORBATE IN THE PURIFICATION OF MUSHROOM TYROSINASE, Phytochemistry, 46(1), 1997, pp. 21-22
Citations number
16
Categorie Soggetti
Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
46
Issue
1
Year of publication
1997
Pages
21 - 22
Database
ISI
SICI code
0031-9422(1997)46:1<21:DITPOA>2.0.ZU;2-H
Abstract
In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the prese nce of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compoun ds in their reduced form throughout the extraction step. Their complet e removal by means of a diafiltration apparatus was achieved. The meth od here described represents a useful way to obtain an enzymic solutio n of tyrosinase, devoid of phenolics and melanic compounds. (C) 1997 E lsevier Science Ltd. All rights reserved.