DETECTION OF ENZYMATIC-HYDROLYSIS PRODUCTS OF SELECTED ACYL AMINE DERIVATIVES BY CHROMATOGRAPHY

Selected hydrolytic enzymes have been used to develop mild conditions for the hydrolysis of acyl derivatives of amines. Either activation or inhibition of enzymes by amines, organic acids or aminoacids used for detecting and determining trace quantities of their compounds. The re sults of determination of the hydrolysis products with the use of oxyd oreductases have been compared with used until now chemical methods. P eroxidase and dehydrogenases such as alcohol (AIDH), lactate (LDH), is ocitrate (ICDH), aldehyde (AdDH) and glutamate (GLDH) make possible to determine amines and organic acids with sensitivities 10-100 times be tter than using chemical methods.