MILLIMETER WAVE-INDUCED VIBRATIONAL-MODES IN DNA AS A POSSIBLE ALTERNATIVE TO ANIMAL TESTS TO PROBE FOR CARCINOGENIC MUTATIONS

Developing methods for alternative testing is increasingly important d ue to dwindling funding resources and increasing costs associated with animal testing and legislation, We propose to test the feasibility of a new and novel method for detecting DNA mutagenesis using millimeter wave spectroscopy, Although millimeter wave spectroscopy has been kno wn since the 1950s, the cost was prohibitive and studies did not exten d to large biological proteins such as DNA, Recent advances have made this technology feasible for developing laboratory and field equipment , We present preliminary findings for lesion-induced vibrational modes in DNA observed from 80 to 1000 gigahertz (GHz). These findings sugge st that there are vibrational modes that can be used as identification resonances, These modes are associated with localized defects of the DNA polymers, They are unique for each defect/lesion, and should be ea sy to detect, We described a field-detecting detector based on the loc al modes. (C) 1997 John Wiley & Sons, Ltd.