AUTOMATED SYNTHESIS OF DOUBLE DYE-LABELED OLIGONUCLEOTIDES USING TETRAMETHYLRHODAMINE (TAMRA) SOLID SUPPORTS

Authors
MULLAH B ANDRUS A
Citation
B. Mullah et A. Andrus, AUTOMATED SYNTHESIS OF DOUBLE DYE-LABELED OLIGONUCLEOTIDES USING TETRAMETHYLRHODAMINE (TAMRA) SOLID SUPPORTS, Tetrahedron letters, 38(33), 1997, pp. 5751-5754
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
33
Year of publication
1997
Pages
5751 - 5754
Database
ISI
SICI code
0040-4039(1997)38:33<5751:ASODDO>2.0.ZU;2-W
Abstract
TAMRA fluorescent dye-labeled non-nucleosidic synthesis supports have been derivatized for automated synthesis of 3'dye-labeled oligonucleot ides.(1) Synthesis with 5' fluorescent dye phosphoramidites leads to 5 ' and 3'double labeled oligonucleotides, useful in Taqman assay and FR ET experiments. Cleavage and deprotection of dye-labeled dye-labeled o ligonucleotides are carried out with tert-butylamine:methnnol:water (1 :1:2) without any degradation and/or modification of dyes and nucleoba ses. (C) 1997 Elsevier Science Ltd.