THE EFFECT OF TEMPERATURE ON THE KINETIC-PROPERTIES OF LACTATE-DEHYDROGENASE FROM EMBRYOS OF VARIOUS FISH SPECIES

Temperature dependence of apparent K-m for pyruvate of LDH from the em bryos of seven species of fishes was analyzed. For the embryos of rain bow trout, autumn cisco and least (Siberian) cisco, the K-m minima wer e observed at 5-8 degrees C; for zebrafish, carp and goldfish they wer e at 25-28 degrees C, and for the loach, at 13-16 degrees C. During em bryonic and larval development of rainbow trout proceeding at graduall y increasing temperatures, the K-m minimum was found to vary in parall el with the optimal temperature. For the embryos of rainbow trout whos e development proceeds at constant temperature, the position of the K- m minimum at the early gastrula stage and at hatching was the same.