DELETION ANALYSIS OF THE SCHISTOSOMA-MANSONI 28-KDA GLUTATHIONE-S-TRANSFERASE GENE PROMOTER IN MAMMALIAN-CELLS - IMPORTANCE OF A PROXIMAL ACTIVATOR-PROTEIN-1 SITE
E. Serra et al., DELETION ANALYSIS OF THE SCHISTOSOMA-MANSONI 28-KDA GLUTATHIONE-S-TRANSFERASE GENE PROMOTER IN MAMMALIAN-CELLS - IMPORTANCE OF A PROXIMAL ACTIVATOR-PROTEIN-1 SITE, European journal of biochemistry, 248(1), 1997, pp. 113-119
The 1241-bp promoter region of the Schistosoma mansoni 28-kDa glutathi
one S-transferase gene (Sm28GST) was sequentially deleted and analyzed
using the Iuciferase reporter gene system in different cell lines. Th
e activator protein-1 (AP-1) site located at -231 seems to be responsi
ble for the major part of the promoter activity. The 1241-bp Sm28GST p
romoter was not, in transient transfection experiments, activated by r
eagents generating reactive oxygen species, such as hydrogen peroxide
(H2O2), 3-methyl-cholanthrene, and ter-methylhydroquinone, but was sig
nificantly stimulated by phorbol 12-myristate 13-acetate, a potent pro
tein kinase C activator. The involvement of the -231 AP-1 site in phor
bol 12-myristate 13-acetate stimulation was demonstrated. Moreover, ev
idence for in vitro and in vivo binding of the -231 AP-1 site to Jun/F
os dimers was obtained using mobility gel shift assays and co-transfec
tion of embryonic F9 cells with Jun/Fos expression plasmids, respectiv
ely. The presence in S. mansoni nuclear extracts of components with af
finity for the AP-1 site suggests conservation of this regulatory path
way in the parasite.