IDENTIFICATION AND CHARACTERIZATION OF AN AADB GENE CASSETTE AT A SECONDARY SITE IN A PLASMID FROM ACINETOBACTER

Authors
SEGAL H ELISHA BG
Citation
H. Segal et Bg. Elisha, IDENTIFICATION AND CHARACTERIZATION OF AN AADB GENE CASSETTE AT A SECONDARY SITE IN A PLASMID FROM ACINETOBACTER, FEMS microbiology letters, 153(2), 1997, pp. 321-326
Citations number
20
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
153
Issue
2
Year of publication
1997
Pages
321 - 326
Database
ISI
SICI code
0378-1097(1997)153:2<321:IACOAA>2.0.ZU;2-E
Abstract
Transformation studies showed that an aminoglycoside resistance gene, aadB, is carried on a 6.0-kb plasmid (pRAY) in a clinical isolate of A cinetobacter (strain SUN). The gene was cloned and sequenced. An analy sis of the DNA sequencing data showed that although the aadB gene is p art of cassette, it is not associated with an integron. Rather, the aa dB cassette has recombined at a secondary site downstream of putative promoters on pRAY.