Pm. Backoff et al., GLYCINERGIC AND GABAERGIC INPUTS AFFECT SHORT-TERM SUPPRESSION IN THECOCHLEAR NUCLEUS, Hearing research, 110(1-2), 1997, pp. 155-163
Most cochlear nucleus (CN) neurons exhibit short-term response suppres
sion to a second stimulus in a paired-pulse (click), forward-masking,
paradigm. The magnitude of suppression, which appears to be greater th
an that observed in acoustic nerve, is dependent on the temporal separ
ation and/or relative intensities of the two stimuli. Recent evidence
suggests that inhibitory circuitry ending on CN neurons may mediate th
is response suppression. Using extracellular recordings from single CN
neurons, suppression was evaluated using a forward-masking paradigm.
Responses to paired acoustic clicks (i.e., a 'masker' followed by an i
dentical 'probe' click) were measured while the time interval between
the masker and probe was varied systematically. The role of inhibitory
circuitry in forward-masking in the CN was assessed by pharmacologic
manipulation of the GABA(A) and glycine(I) (strychnine-sensitive) rece
ptors. Blockade of glycinergic or GABAergic receptors by iontophoretic
application of the antagonists, strychnine and bicuculline methiodide
, decreased the effects of forward-masking by shortening recovery time
s of the probe response in 2/3 of the neurons tested. Conversely, agon
ist application (glycine, and GABA or muscimol) increased the magnitud
e of suppression and delayed recovery of the probe response relative t
o control values. These findings suggest that known circuits releasing
glycine and/or GABA mediate short-term response suppression in some C
N neurons.