GLYCERALDEHYDE 3-PHOSPHATE-INDUCED DNA OR PROTEIN MODIFICATIONS SEVERELY INHIBIT THE PROTEIN DNA INTERACTION/

In this study, the effect of the reducing sugar glyceraldehyde 3-phosp hate on protein/DNA interaction has been investigated. Treatment with glyceraldehyde 3-phosphate of oligonucleotides recognized by various t ranscription factors severely inhibits protein binding. The inhibitory effect is time and dose-dependent. Treatment with glyceraldehyde 3-ph osphate of the homeodomain protein TTF-1 HD has also an inhibitory eff ect on the interaction with DNA, again in a time and dose-dependent ma nner. These ''in vitro'' effects could have ''in vivo'' counterparts a nd therefore contribute to molecular alterations observed either when intracellular protein are exposed to high doses of reducing sugars (i. e. in diabetes) or after a long time exposure (i.e. in G(0)-arrested c ells during aging).