COUPLING OF MUSCLE PHOSPHORYLATION POTENTIAL TO GLYCOLYSIS DURING WORK AFTER SHORT-TERM TRAINING

To examine whether the metabolic adaptations to short-term training ar e expressed over a range of submaximal levels of mitochondrial respira tion, seven untrained male subjects [maximal O-2 uptake (VO2 max ) = 4 5.9 +/- 1.9 (SE) ml.kg(-1).min(-1)] performed a progressive three-stag e protocol of cycle exercise at 60% (20 min), 79% (20 min), and 92% (1 1 min) of pretraining VO2 max before and after training. Training cons isted of 5-6 days of cycling for 2 h/day at 65% VO2 max . Muscle tissu e rapidly obtained from the vastus lateralis by needle biopsy indicate d that training blunted (P < 0.05) the increase in lactate observed at 60% (23.4 +/- 6.5 vs. 12.4 +/- 2.9 mmol/kg dry wt), 79% (48.9 +/- 5.1 vs. 25.6 +/- 5.2 mmol/kg dry wt), and 92% (68.3 +/- 6.4 vs. 41.5 +/- 6.5 mmol/kg dry wt) of VO2 max . Training also resulted in a higher ph osphocreatine and lower creatine and P-i concentrations at both 79% (P < 0.05) and 92% (P < 0.05) of VO2 max and higher muscle glycogen leve ls (P < 0.05). These changes were accompanied by small but significant reductions (P < 0.05) in O-2 uptake at the two higher exercise intens ities. Given that the lactate-to-pyruvate ratio and the calculated fre e ADP and AMP were also reduced (P < 0.05), it would appear that short -term training results in a tighter metabolic control over a range of mitochondrial respiratory rates.