DETECTION OF TRANSITION-METALS DURING THEIR SEPARATION IN AN ISOCONDUCTIVE PH GRADIENT

The possibility of the conductimetric detection of transition metals f ollowing separation with a linear DH gradient was demonstrated. Such d etection is possible owing to the constant conductivity of the polyamp holyte eluent over a wide pH range due to the use of a starting buffer with the same conductivity as the polyampholyte eluent. The separatio n of metals was performed on a column packed with tetraethylenepentami ne-bonded silica.