PURIFICATION OF BACILLI RIBONUCLEASES BY REVERSED-PHASE HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY

A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucle ases. A double gradient system of elution is used. Initial concentrati on of the sample followed by reversed-phase HPLC gives high yields (90 -95%) of homogeneous, active protein on both analytical and preparativ e scales. The procedure may be applied to the isolation of ribonucleas es from different sources without significant modifications