P. Husek et Hm. Liebich, ORGANIC-ACID PROFILING BY DIRECT TREATMENT OF DEPROTEINIZED PLASMA WITH ETHYL CHLOROFORMATE, Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 37-43
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
Carboxylic acids in plasma or serum can be conveniently determined by
capillary gas chromatography (GC) following treatment with ethyl chlor
oformate (ECF). The mixed organic solvent used for plasma deproteiniza
tion is also suitable as medium for the subsequent reaction step. Thus
, isolation of the compounds of interest is not necessary. Before trea
tment of the supernatant with ECF, the neutral lipids and amino acids
are removed easily by hexane extraction and cation-exchange chromatogr
aphy. Ketocarboxylic acids do not require a preliminary oximation. Cap
illary columns with a length of 15 m and a polar silicone phase proved
to be ideal for the separation of mixtures of derivatized keto-, hydr
oxy-, mono- and dicarboxylic acids. The run time is less than 30 min.