B. Trumbach et al., IMPROVED SEPARATION OF RADIOACTIVELY LABELED CELLULAR PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 73-76
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
An improved high-performance liquid chromatographic method for the sep
aration and determination of radioactively labelled cellular phospholi
pids is described. The method is based on separation of phospholipids
on a 250 x 4 mm I.D. LiChrospher DIOL 100 (5 mu m) column, fitted with
a 50 x 4 mm I.D. LiChrospher Si 60 (5 mu m) precolumn and a gradient
of 5% H3O4 and acetonitrile. It allows the determination of small amou
nts of labelled phosphatidylcholine and sphingomyelin due to the sharp
elution profile in spite of long retention times.