IMPROVED SEPARATION OF RADIOACTIVELY LABELED CELLULAR PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
TRUMBACH B ROGLER G LACKNER KJ SCHMITZ G
Citation
B. Trumbach et al., IMPROVED SEPARATION OF RADIOACTIVELY LABELED CELLULAR PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 73-76
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
656
Issue
1
Year of publication
1994
Pages
73 - 76
Database
ISI
SICI code
Abstract
An improved high-performance liquid chromatographic method for the sep aration and determination of radioactively labelled cellular phospholi pids is described. The method is based on separation of phospholipids on a 250 x 4 mm I.D. LiChrospher DIOL 100 (5 mu m) column, fitted with a 50 x 4 mm I.D. LiChrospher Si 60 (5 mu m) precolumn and a gradient of 5% H3O4 and acetonitrile. It allows the determination of small amou nts of labelled phosphatidylcholine and sphingomyelin due to the sharp elution profile in spite of long retention times.