EDMA-2000 AS A MATRIX FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF HUMAN HEMOGLOBIN CHAINS

Authors
SUTTNAR J FORTOVA H BRABEC V
Citation
J. Suttnar et al., EDMA-2000 AS A MATRIX FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF HUMAN HEMOGLOBIN CHAINS, Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 119-122
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
656
Issue
1
Year of publication
1994
Pages
119 - 122
Database
ISI
SICI code
Abstract
An ethylenedimethacrylate polymer-based matrix (EDMA 2000) is describe d that shortens the time for separation of haemoglobin (Hb) chains to 30 min, compared with the use of C-4 large-pore Vydac columns, commonl y used for reversed-phase HPLC of Hb variants. EDMA 2000 was successfu lly used for the isolation of an abnormal beta-chain of the unstable H b recently characterized as Hb Nottingham.