MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY OF 8-HYDROXYDEOXYGUANOSINE ACID OTHER OXIDIZED DERIVATIVES OF DNA

8-Hydroxydeoxyguanosine (8-OH-dG) is widely recognized as a marker of DNA oxidation. Until now, 8-OH-dG has been measured by high-performanc e liquid chromatography or by gas chromatography-mass spectrometry. A method is reported that detects oxidative derivatives of deoxynucleosi des by micellar electrokinetic capillary chromatography. Single-strand ed DNA was incubated in the presence of 50 mM hydrogen peroxide-10 mM ascorbic acid and hydrolysed by enzymatic digestion. The order of elec trophoretic mobilities of deoxynucleosides was dC>dA>T>dG>8-OH-dG. 8-O H-dG was determined by introducing a laboratory-prepared internal stan dard. Two additional major oxidative derivatives were identified by co mparing the electropherogram of the oxidized DNA with that of the oxid ized standard deoxyguanosine.