SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF THE 3 MAIN METABOLITES OF SELEGILINE (L-DEPRENYL) IN HUMAN PLASMA

Authors
LACROIX R PIANEZZOLA E BENEDETTI S
Citation
R. Lacroix et al., SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF THE 3 MAIN METABOLITES OF SELEGILINE (L-DEPRENYL) IN HUMAN PLASMA, Journal of chromatography B. Biomedical applications, 656(1), 1994, pp. 251-258
Citations number
38
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
656
Issue
1
Year of publication
1994
Pages
251 - 258
Database
ISI
SICI code
Abstract
A high-performance liquid chromatographic (HPLC) method with fluoresen ce detection was developed for the determination in human plasma of th e three main metabolites of selegiline (L-deprenyl): amphetamine, meth amphetamine and norselegiline. The HPLC separation of the analytes was performed under isocratic conditions, after extraction from plasma an d precolumn derivatization with 9-fluorenylmethyl chloroformate. The l inearity, precision and accuracy of the method were evaluated; the lim it of quantification for all three metabolites in plasma was 0.5 ng/ml .