ELICITOR-INDUCED FORMATION OF FREE AND CELL-WALL-BOUND STILBENES IN CELL-SUSPENSION CULTURES OF SCOTS-PINE (PINUS-SYLVESTRIS L)

Treatment of Pinus sylvestris L. cell-suspension cultures with an elic itor preparation from the pine needle pathogen Lophodermium seditiosum , resulted in a several-hundred- to thousandfold accumulation of the s tilbenes pinosylvin and pinosylvin 3-O-methyl ether in methanolic cell extracts. There was a simultaneous induction of the biosynthetic enzy mes phenylalanine ammonia-lyase (E.C. 4.3.1.5.) and stilbene synthase (pinosylvin-forming, E.C. 2.3.1.146). For the first time, an incorpora tion of stilbenes into the cell wall fraction as well as stilbene excr etion into the extracellular space was demonstrated in addition to int racellular accumulation.