Mt. Gonzalezgarza et S. Saidfernandez, IN-VITRO ANTI-TRICHOMONAD EFFECTIVENESS OF A GOSSYPOL-METRONIDAZOL BLEND, International journal of antimicrobial agents, 9(1), 1997, pp. 57-60
With the aid of the Basic Sequential Analytical Simplex Method the in
vitro anti-trichomonad effectiveness of several metronidazole/gossypol
blends was determined. While the combination that produced 50% of gro
wth inhibition on trichomonad cultures (IC50) contained 15.1 mu M goss
ypol and 0.087 mu M metronidazole, the IC50 of metronidazole and gossy
pol tested separately was 38.08 mu M and 0.195 mu M, respectively. Thu
s, the IC50 of mixed gossypol and metronidazole was, respectively 60.3
4 and 55.4% lower than that of each separated drug. Whereas metronidaz
ole is usually administered orally, gossypol, used as spermicide, is f
ormulated as pessary. In such conditions the absorption of metronidazo
le is very efficient while that of gossypol is strikingly poor. The ab
ove suggest that a gossypol-metronidazole anti-trichomoniasis therapeu
tic scheme could be as effective as metronidazole alone, but less toxi
c. (C) 1997 Elsevier Science B.V.