STUDIES ON THE EXPRESSION OF MARKER GENES IN CHICKPEA

Citation
T. Husnain et al., STUDIES ON THE EXPRESSION OF MARKER GENES IN CHICKPEA, Plant cell, tissue and organ culture, 49(1), 1997, pp. 7-16
Citations number
20
Categorie Soggetti
Plant Sciences
ISSN journal
01676857
Volume
49
Issue
1
Year of publication
1997
Pages
7 - 16
Database
ISI
SICI code
0167-6857(1997)49:1<7:SOTEOM>2.0.ZU;2-9
Abstract
Conditions were established for the optimum transient expression of be ta-glucuronidase and neomycin phosphotransferase II genes introduced i nto zygotic embryos of chickpea (Cicer arietinum L. 6153 and CM72) by accelerated tungsten particles. Plasmid DNA at a concentration of 12 m icrogram per milligram of tungsten particles when accelerated with an inflow of helium gas at 60 kilogram per square centimeter through a di stance of 24 centimeter in a chamber maintained at a negative pressure of 71.12 centimeter of mercury, resulted in optimal transient express ion of the beta-glucuronidase gene in chickpea embryos. However, the e xpression of the marker genes was 20-40% higher under a cauliflower mo saic virus promoter in comparison to the Win6 and actin promoters. Whe n Agrobacterium tumefaciens was used to transfer marker genes into zyg otic embryos and the resultant plants were analysed for activity of th e beta-glucuronidase and neomycin phosphotransferase II genes, both of these genes were expressed in tumorous tissues. When a disarmed strai n of Agrobacterium was used, normal shoots were regenerated in which t he lower parts showed expression of both genes at a frequency of 20%.