EFFECTS OF LOW-TEMPERATURE ON INDUCTION AND DIFFERENTIATION OF WHEAT CALLUSES

Young inflorescences and long-term culture calluses of wheat (Triticum aestivum L.) were used to investigate the effect of low temperature t reatment on the potential of plant regeneration. The results indicated that the frequency of callus induction from immature inflorescences w as decreased when treated for a long time at 5 degrees C. However, it was found that a 5 degrees C treatment significantly improved the rege neration frequency of calluses. The clear difference of peroxidases of wheat calluses was apparent after the cultures were treated at the lo w temperature. The isozyme band 8 became clearly faint, however, bands 6 and 9 became intense.