EFFECTIVENESS OF INDOLEACETIC-ACID, INDOLEBUTYRIC-ACID AND NAPHTHALENEACETIC-ACID DURING ADVENTITIOUS ROOT-FORMATION IN-VITRO IN MALUS-JORK-9

Citation
Gj. Deklerk et al., EFFECTIVENESS OF INDOLEACETIC-ACID, INDOLEBUTYRIC-ACID AND NAPHTHALENEACETIC-ACID DURING ADVENTITIOUS ROOT-FORMATION IN-VITRO IN MALUS-JORK-9, Plant cell, tissue and organ culture, 49(1), 1997, pp. 39-44
Citations number
16
Categorie Soggetti
Plant Sciences
ISSN journal
01676857
Volume
49
Issue
1
Year of publication
1997
Pages
39 - 44
Database
ISI
SICI code
0167-6857(1997)49:1<39:EOIIAN>2.0.ZU;2-G
Abstract
We have examined in vitro rooting of apple 'Jork 9' shoots exposed for three weeks to each of the three auxins commonly used for ex vitro ro oting: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and alp ha-naphthaleneacetic acid (NAA). During the initial five days of the r ooting treatment, the cultures were incubated in darkness. In this per iod, the root initials are formed. Then, the cultures were moved to th e Light. NAA resulted in a low (ca. 8 roots), and IAA or IBA in a high (ca. 15 roots) maximal root number. The maximal root number was reach ed at a wide range of IAA concentrations (10-100 mu M) but at only one concentration of IBA (10 mu M) or NAA (3 mu M). With NAA and IBA, gro wth of roots and shoots was much more inhibited than with IAA. For the se reasons, IAA is the preferable auxin for in vitro rooting of apple 'Jork 9' shoots.