NUCLEOTIDE-SEQUENCE OF PI CLASS GLUTATHIONE-S-TRANSFERASE IN HUMAN FETAL LIVER

The GST Fpi and GST Ppi, pi class glutathione S-transferase (GST), wer e purified from human fetal livers and placentas, respectively. Both G ST enzymes were indistinguishable each other in their subunit molecula r weights, immunochemical properties and substrate specificities. Thre e clones (pFGP-1, pFGP-2 and pFGP-3) coding for the pi class GST purif ied from fetal livers were isolated from a human fetal liver cDNA libr ary. The full-length clone encodes a polypeptide comprising 210 amino acid including the initiator methionine. All of these cDNA clones were nearly identical to a human placental cDNA clone, pGpi 2. The pFGP-1 cDNA had only a single base transition accompanied by an amino acid tr ansition in the coding region, at position 313. The pFGP-2 and pFGP-3 cDNAs were also nearly identical to pGpi 2 cDNA, having only a single silent C --> T transition in the coding region, at position 555.