A truncated cryIA(b) gene encoding the active region of the Bacillus t
huringiensis delta-endotoxin was expressed in transgenic sugarcane pla
nts (Saccharum officinarum L.) under the control of the CaMV 35S promo
ter. Genetic transformation was accomplished by electroporation of int
act cells. The levels of recombinant toxin were established and biolog
ical activity tests were performed against neonate sugarcane borer (Di
atraea saccharalis F.) larvae. Transgenic sugarcane plants showed sign
ificant larvicidal activity despite the low expression of CryIA(b).