DETERMINATION OF BIOGENIC-AMINES AND RELATED-COMPOUNDS IN THE GANGLIAAND THE AURICLE AND VENTRICLE OF THE HEART OF THE SNAIL HELIX-LUCORUML BY HPLC WITH AMPEROMETRIC DETECTION
A. Pappalouisi et al., DETERMINATION OF BIOGENIC-AMINES AND RELATED-COMPOUNDS IN THE GANGLIAAND THE AURICLE AND VENTRICLE OF THE HEART OF THE SNAIL HELIX-LUCORUML BY HPLC WITH AMPEROMETRIC DETECTION, Journal of liquid chromatography & related technologies, 20(15), 1997, pp. 2427-2439
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Biogenic amines and related compounds in the suboesophageal ganglia an
d the auricle and ventricle of the heart of the snail Helix lucorum L.
have been identified and quantified using the technique of reversed p
hase ion-pair HPLC with amperometric detection. HPLC analysis revealed
the presence of two catecholamines, Dopamine (DA) and Norepinephrine
(NE), one indoleamine, Serotonin (5HT), its immediate precursor 5-Hydr
oxytryptophan (5HTP) and their metabolites 3,4-Dihydroxy phenylacetic
acid (DOPAC)and 5-Hydroxindole-3-acetic acid (5HIAA). Quantitatively,
DA and SI-FT were the most prominent monoamines detected, with the gan
glia containing the highest concentrations of both. The ganglia contai
ned also assayable amounts of NE and DOPAC.