Aw. Probert et al., SODIUM-CHANNEL MODULATORS PREVENT OXYGEN AND GLUCOSE DEPRIVATION INJURY AND GLUTAMATE RELEASE IN RAT NEOCORTICAL CULTURES, Neuropharmacology, 36(8), 1997, pp. 1031-1038
Neocortical cultures were deprived of oxygen and glucose to model isch
emic neuronal injury. We used a graded series of periods of oxygen and
glucose deprivation, providing graded insults. Cell death was measure
d by release of lactate dehydrogenase (LDH). One hundred and twenty to
240 min of deprivation caused graded increases in glutamate overflow,
LDH release and Ca-45 influx. Curves of LDH release with respect to d
eprivation time were shifted to longer intervals by treatment with tet
rodotoxin (TTX; 3, 30 or 300 nM), phenytoin (10, 30 or 100 mu M), lido
caine (10, 30 or 100 mu M) or the N-methyl-D-aspartate antagonist CPP
[3(2-carboxypiperazine-4-yl)propyl-1-phosphonic acid, 3, 10, 30 or 100
mu M]. Combined treatment with TTX and CPP caused pronounced rightwar
d shifts of LDH deprivation curves. Our results indicate that Na+ chan
nel blockade is neuroprotective in neocortex cultures. Our results als
o suggest that neuroprotection with Na+ channel blockers may be due to
inhibition of glutamate release. (C) 1997 Published by Elsevier Scien
ce Ltd.