PLANT-REGENERATION FROM CALLUS-CULTURES OF PSORALEA-CORYLIFOLIA LINN

Plantlet regeneration via organogenesis was achieved in callus culture s derived form mature leaves, stems and leaves, petioles and roots of young seedling of Psoralea corylifolia on Murashige and Skoog medium s upplemented with 2.5-3.0 mg L-1 BA, 1.0 mg L-1 NAA and 3% (w/v) sucros e. The rate of shoot bud regeneration was positively correlated with t he concentration of hormones in the nutrient media. Shoot buds regener ated more readily from juvenile explants (seedling source) as compared to the mature explants. Addition of adenine sulphate (5 mg L-1) to th e culture medium increased the growth of shoot buds. Optimum responses were obtained in hypocotyl and leaf explants using NAA in combination with BA, the highest rate of shoot bud regeneration being in hypocoty l explants. Rooting was readily achieved on the differentiated shoots on MS basal media without growth regulators. Regenerated plantlets wer e successfully established in the greenhouse.