PROTON AND PHOSPHORUS NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF HUMAN BRAIN-TUMOR EXTRACTS WITH AUTOMATIC DATA CLASSIFICATION - A PRELIMINARY-STUDY

High-resolution one-dimensional proton and phosphorus and two dimensio nal COSY proton Magnetic Resonance Spectroscopy were used to investiga te the lipid and carbohydrate metabolism of human brain tumors. Sixtee n meningioma (MG) (benign tumors) and ten glioblastoma (GB) (malignant tumors) samples from brain surgery were treated for dual extraction o f lipidic and aqueous phases before NMR processing. A highly significa nt variation of the H-1 metabolite spectral pattern was observed betwe en benign and malignant tumors. Double extraction method combined with both H-1 and P-31 NMR in vitro analyses provided a large set of bioch emical information which may be statistically analyzed to elucidate tu mor-specific biochemical pathways and to improve interpretation of in vivo spectra.