TOWARDS THE DEVELOPMENT OF A BIOARTIFICIAL PANCREAS - A C-13 NMR-STUDY ON THE EFFECTS OF ALGINATE POLY-L-LYSINE/ALGINATE ENTRAPMENT ON GLUCOSE-METABOLISM BY BETA-TC3 MOUSE INSULINOMA CELLS/
I. Constantinidis et al., TOWARDS THE DEVELOPMENT OF A BIOARTIFICIAL PANCREAS - A C-13 NMR-STUDY ON THE EFFECTS OF ALGINATE POLY-L-LYSINE/ALGINATE ENTRAPMENT ON GLUCOSE-METABOLISM BY BETA-TC3 MOUSE INSULINOMA CELLS/, Cellular and molecular biology, 43(5), 1997, pp. 721-729
We have utilized C-13 NMR spectroscopy to investigate glucose metaboli
sm in the mouse insulinoma beta TC3 cell line. Cells were cultured and
examined both as monolayers, and entrapped in alginate/poly-L-lysine/
alginate beads. Entrapped cultures were tested at 3 and 30 days post-e
ntrapment. The purpose of this study was to assess whether the entrapp
ed environment affects glucose metabolism and insulin secretion. Both
monolayer and entrapped cultures were fed with 10 mM [1-C-13]-glucose
for 4 hrs. prior to extraction with perchloric acid. Our data revealed
that beta TC3 cells possessed a reduced tricarboxylic acid (TCA) acti
vity in the entrapped cultures, and that they metabolized pyruvate pri
marily via pyruvate dehydrogenase regardless of the mode or age of the
culture.