RAS P21 IMMUNOHISTOCHEMICAL DETECTION IN HUMAN ENDOMETRIAL CARCINOMAS

Tissue samples of 40 patients with histologically confirmed endometria l cancer were analyzed immunohistochemically on paraffin-embedded spec imens to detect ras p21 protein expression. The relationship between p 21 protein expression and clinicopathological findings was also analyz ed. The intensity and distribution of specific cytoplasmatic staining were evaluated semiquantitatively by counting the immunohistochemical H-score. ras p21 expression was found in 30 (75%) of 40 human endometr ial carcinomas, regardless of the clinical stage of the disease. Posit ive immunostaining for p21 was noted in 68% of stage I-II and in all 8 of the advanced stages (III-IV according to PICO) of endometrial carc inomas. Myometrial invasion was related to ras p21 immunostaining (p = 0.009), however, no correlation between histological findings and ms p21 expression was observed.