SURVIVAL OF AN UNIRRADIATED SALMONELLA-TYPHIMURIUM MARKER STRAIN INOCULATED IN POULTRY FEEDS AFTER IRRADIATION

The present study was designed to compare unirradiated Salmonella typh imurium survival during storage after inoculation in either irradiated or unirradiated poultry feed. The effects of irradiation (5 kGy) on t he indigenous feed microflora and on the survival of marker strain of S. typhimurium contaminated after irradiation treatment were determine d during 56 days of storage of either soybean meal (SBM) or meat and b one meal (MBM) based feeds. The initial aerobic bacterial populations were reduced more than 90% in both SBM (4,96 to 4.08 +/- 0.03 log(10) CFU/g feed) and MBM (5.12 to 3.90 +/- 0.03) by irradiation. Irradiatio n treatment reduced the average fungal counts during 56 days of storag e in both SBM (4.24 to 2.74 +/- 0.03) and MBM (4.38 to 2.15 +/- 0.03) containing feeds. However, unirradiated S. typhimurium populations ino culated after irradiation of the feed were not different in either irr adiated or nonirradiated SBM and MBM based feeds. Therefore, the diffe rences in fungal versus bacterial sensitivity among the feed types and storage times suggests that gamma irradiation can alter the makeup of indigenous microbial populations in feed but this does not appear to have a discernible influence on subsequent survival of unirradiated S. typhimurium added as a dry inoculum after irradiation.