HYDROLYTIC METALLOENZYME ACTIVITY OF ZINC (II) COMPLEX OF TRIS(2-BENZIMIDAZYLMETHYL)AMINE

The hydrolytic kinetics of p-nitrophenol acetate(NA) catalyzed by zinc ( II) complex of Tris (2-benzimidazylmethyl)amine (NTB) has been studi ed. It was shown that the catalytic hydrolytic rate is of first-order to both zinc( II) complex and NA concentrations. The second-order rate constant is k(cat) = 0.12 mol(-1).L.s(-1)(T=298 K, I = 0.10 mol/L KNO 3, 0.02 mol/L Tris buffer, 40% CH3CN aqueous solution). This is one of the most efficient hydrolytic metalloenzyme model compounds reported up to now, The increase of CH3CN contents in the solution greatly decr eased the hydrolytic activity of the nucleophiles.