OXYGENATION CAPABILITIES OF BASKET-TYPE BIOREACTORS FOR MICROCARRIER CULTURES OF ANCHORAGE-DEPENDENT CELLS

Authors
KALOGERAKIS N BEHIE LA
Citation
N. Kalogerakis et La. Behie, OXYGENATION CAPABILITIES OF BASKET-TYPE BIOREACTORS FOR MICROCARRIER CULTURES OF ANCHORAGE-DEPENDENT CELLS, Bioprocess engineering, 17(3), 1997, pp. 151-156
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Bioprocess engineering → ACNP
ISSN journal
0178515X
Volume
17
Issue
3
Year of publication
1997
Pages
151 - 156
Database
ISI
SICI code
0178-515X(1997)17:3<151:OCOBBF>2.0.ZU;2-2
Abstract
The oxygenation capabilities of a new generation three phase - two reg ion 1200 l bioreactor employed for the cultivation of anchorage depend ent animal cells were investigated experimentally. A mathematical mode l has also been developed that explains qualitatively the observed oxy genation characteristics. This type of bioreactor, that uses microcarr ier support particles, has two distinct mesh-separated regions so that the air bubbles in the oxygenation region do not come into contact wi th the microcarriers in the cell (bubble-free) region. Implications on achievable maximum cell densities are also discussed.