A NEW RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM FROM CRYPTOSPORIDIUM-PARVUM IDENTIFIES GENETICALLY HETEROGENEOUS PARASITE POPULATIONS AND GENOTYPIC CHANGES FOLLOWING TRANSMISSION FROM BOVINE TO HUMAN HOSTS

Authors
CARRAWAY M TZIPORI S WIDMER G
Citation
M. Carraway et al., A NEW RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM FROM CRYPTOSPORIDIUM-PARVUM IDENTIFIES GENETICALLY HETEROGENEOUS PARASITE POPULATIONS AND GENOTYPIC CHANGES FOLLOWING TRANSMISSION FROM BOVINE TO HUMAN HOSTS, Infection and immunity, 65(9), 1997, pp. 3958-3960
Citations number
7
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
65
Issue
9
Year of publication
1997
Pages
3958 - 3960
Database
ISI
SICI code
0019-9567(1997)65:9<3958:ANRFC>2.0.ZU;2-E
Abstract
Length and restriction site polymorphism within a 2.8-kb threonine-ric h open reading frame from Cryptosporidium parvum was identified and us ed to determine the genotypes of isolates from calves and humans, In a greement with observations of other genetic loci, all calf isolates we re identical at this locus. In contrast, human isolates showed two pro files, one found exclusively in humans and one a superposition of both profiles, which were indicative of heterogeneous parasite populations . PCR fingerprints were consistent with a change in the genetic profil e of C. parvum isolates following transmission from bovine to human ho sts.